Genistein inhibits the proliferation of human choriocarcinoma cells via the downregulation of estrogen receptor-? phosphorylation at serine 118

•Methyl-piperidino-pyrazole downregulated phosphorylated estrogen receptor-? (S118).•Genistein further decreased the levels of receptor-?.•Genistein reduced proliferation JEG-3 human choriocarcinoma cells.•Genistein Ki-67 in cells. Background & aimsChoriocarcinoma is a malignant trophoblastic tumor. The phosphorylation at serine 118 (p-ER-s118) decreases cancer cell proliferation. However, effect genistein as modulator p-ER-s118 and chorioarcinoma cells remains to be understood. This study aims determining function on proliferation.MethodsAfter reaching confluency, were divided into six groups, control group (without methyl-piperidino-pyrazole (MPP) pre-treatment treatment); groups with treated concentrations 0, 10, 25, 50, 100 ?M (cells pretreated MPP). Expression analyzed using immunocytochemistry.ResultsDifferent doses compared those (p < 0.05). was inhibited by MPP pre-treatment, concomitant treatment dose 0 ?M, 25 50 0.05).ConclusionTaken together, p-ER-s118. addition Thus, vivo studies nedd follow this vitro elucidate mechanism(s) employed an alternative therapy for choriocarcinoma. Choriocarcinoma After immunocytochemistry. Different Taken